LOCUS       40924_1414        6086 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Gene silencing vector ipSilent DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6086)
  AUTHORS   Vu BV, Pham KT, Nakayashiki H.
  TITLE     Substrate-Induced Transcriptional Activation of the MoCel7C 
            Cellulase Gene Is Associated with Methylation of Histone H3 at 
            Lysine 4 in the Rice Blast Fungus Magnaporthe oryzae
  JOURNAL   Appl. Environ. Microbiol. 79 (21), 6823-6832 (2013)
  PUBMED    23995923
REFERENCE   2  (bases 1 to 6086)
  AUTHORS   Nakayashiki H, Vu BV.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2012) Contact:Hitoshi Nakayashiki Kobe University,
            Faculty of Agriculture; 1-1 Rokkodai, Nada, Kobe, Hyogo 657-8501, 
            Japan
REFERENCE   3  (bases 1 to 6086)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6086)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2013"; volume: "79"; issue: "21"; 
            pages: "6823-6832"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-SEP-2012) Contact:Hitoshi Nakayashiki Kobe University, Faculty 
            of Agriculture; 1-1 Rokkodai, Nada, Kobe, Hyogo 657-8501, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6086
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /label=AmpR promoter
     CDS             129..986
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1160..1748
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2072..2102
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2234..2588
                     /label=trpC promoter
                     /note="promoter for Aspergillus nidulans trpC"
     CDS             2593..3615
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     regulatory      3619..3864
                     /label=Aspergillus nidulans TrpC terminator
                     /note="Aspergillus nidulans TrpC terminator"
                     /regulatory_class="terminator"
     primer_bind     3873..3889
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      3892..4990
                     /label=Magnaporthe oryzae MoCel7C promoter
                     /note="Magnaporthe oryzae MoCel7C promoter"
                     /regulatory_class="promoter"
     misc_feature    4991..5028
                     /label=multi-cloning sites
                     /note="multi-cloning sites"
     intron          5029..5162
                     /note="Magnaporthe oryzae cutinase intron2"
     misc_feature    5163..5228
                     /label=multi-cloning sites
                     /note="multi-cloning sites"
     regulatory      5229..5432
                     /label=Magnaporthe oryzae MoCel7C terminator
                     /note="Magnaporthe oryzae MoCel7C terminator"
                     /regulatory_class="terminator"
     promoter        complement(5443..5461)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5471..5487)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      5629..6084
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctccac cgcggtggcg
     2221 gccgcgacgt taactgatat tgaaggagca ctttttgggc ttggctggag ctagtggagg
     2281 tcaacaatga atgcctattt tggtttagtc gtccaggcgg tgagcacaaa atttgtgtcg
     2341 tttgacaaga tggttcattt aggcaactgg tcagatcagc cccacttgta gcagtagcgg
     2401 cggcgctcga agtgtgactc ttattagcag acaggaacga ggacattatt atcatctgct
     2461 gcttggtgca cgataacttg gtgcgtttgt caagcaaggt aagtgaacga cccggtcata
     2521 ccttcttaag ttcgcccttc ctccctttat ttcagattca atctgactta cctattctac
     2581 ccaagcatcg atatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc
     2641 gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct
     2701 ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt
     2761 ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa
     2821 gtgcttgaca ttggggaatt cagcgagagc ctgacctatt gcatctcccg ccgtgcacag
     2881 ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg
     2941 gaggccatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc
     3001 ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat
     3061 ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag
     3121 gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac
     3181 gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc ggtcattgac
     3241 tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg
     3301 ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca tccggagctt
     3361 gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg gtcttgacca actctatcag
     3421 agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc
     3481 gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc
     3541 tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc cagcactcgt
     3601 ccgagggcaa aggaatagag tagatgccga ccgggatcca cttaacgtta ctgaaatcat
     3661 caaacagctt gacgaatctg gatataagat cgttggtgtc gatgtcagct ccggagttga
     3721 gacaaatggt gttcaggatc tcgataagat acgttcattt gtccaagcag caaagagtgc
     3781 cttctagtga tttaatagct ccatgtcaac aagaataaaa cgcgtttcgg gtttacctct
     3841 tccagataca gctcatctgc aatggcggcc gctctagaac tagtggatcc ccctagtgat
     3901 ggtggtattg ttgagggttg gcactgccaa catgagaagg ccaagcacaa gatactttga
     3961 agatcaatga tgtaacagag taggatacaa gcggtagtaa tcataactga ctttccacga
     4021 ttgggtgtct acagccgtga tggagtgttt cggggtagca atagtatatc gccgtccccg
     4081 cagctggtgc cacgccagtc ggaaccagat ttatcacagc ccgacccgtg aaaacggctg
     4141 atcaataagt aaacctattc caaattcgat gtttccaagc cggacttaac cgcactgggg
     4201 taagcgtctt cagctcgaca ggggtttcat tgacagacgg ggaaatggca atataggccc
     4261 gccaaccggg acacgtctgg gttcaggatt ggatggactg aaaggagcaa aaagacaaaa
     4321 aaagttgttg catgaacagg cccatactct atactctatt ctgactgccc cggaatcacg
     4381 ggcccgtctt tgccccttgg cctctgccac acctttttcc cgccatcccg actccaagga
     4441 cgggagacga ccttccgcca atccaatgca gcaagtccac cattcttggc gtgcggagga
     4501 tggggtagtt gtgcggaggt cagatttctt ttttccgatc tggagagaag gggctttttt
     4561 tgctgcaatc acggctaatg tgcagcgagc cgatattcca atttccaaga tttttttttt
     4621 tcaactggcg agccgcggtc gcgctggctt tccgcttccc caaccgagat gtgtgccttg
     4681 catgtggtta ggtatagctg gccgtgaggt aattcgatcc tgtgattggg ttttagtgcc
     4741 caatgggtaa ggggatgaat tggacgtgtg gggaagacgt gaatgaaccg atccgaatat
     4801 tcggcgagta taaacaggaa tagtcgcccg cctggctgta atctatcgtg gctcttcacc
     4861 tcagcccctt aatatacgag gcccatcttt ctgtcgtctt gcccgtctga cagctttttt
     4921 tcctcagacc ctggagcagc agtccgtccc cgtccaacgc cccccccccc cccaacgcca
     4981 gccaaagaaa atgcatgaat tcgatatcaa gcttgctgga ggatacaggt gagcctttct
     5041 tcttgcctct ctttgttttt tttttgttct ttttgccgaa tagtgtaccc actggagatt
     5101 tgttggccat gcaaataaat ggaagggact gacaagattg tgaaattgtt caaaacacac
     5161 agccagggaa cggcagatct tcgcatgcta aggcctgtgg tacctgggcc ccggatccac
     5221 ttgtcgacct gggaggttgt tgatgtggaa ctcggaggtt gagagtgtga tggcattaaa
     5281 tacattgatc tgaatggaga gatctagggg gtattctaaa gcatacaaaa tggaacagta
     5341 cctacactga agccatctca ggaaataaaa caaaataaat gtacaagcac cgccaagttt
     5401 atacgtgggc ccgggacctc gagggggggc ccgccaattc gccctatagt gagtcgtatt
     5461 acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     5521 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     5581 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta
     5641 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     5701 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     5761 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     5821 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     5881 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     5941 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     6001 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     6061 acaaaatatt aacgcttaca atttag
//
